Richard P. Visconti, Ph.D.
Room 605A, Children's Research Institute
Office: (843) 792-6024
Education:BS Ursinus College, Collegeville, PA, 1988 Biology
MA Temple University, Philadelphia, PA, 1992 Developmental Biol.
PhD Temple University, Philadelphia, PA, 1995 Developmental Biol.
Research Interests:Certain organs, such as the heart, lack inherent mechanisms for functional recovery after injury. With this in mind, the overall research focus in the Visconti lab is on elucidation of the cellular and molecular mechanisms through which endogenous stem cell populations contribute to both the embryonic morphogenesis of cardiovascular tissues and to their pathological remodeling following injury or disease. Our belief is that a fundamental understanding of how stem cells contribute to these natural functions will provide insights that can be applied to regenerative interventions. Our lab currently focuses on two projects related to this theme:
- Elucidation of the contribution of hematopoietic stem cells to cardiac morphogenesis during embryonic development and to pathological remodeling of the heart in disease: Hematopoietic stem and progenitor cells were traditionally thought to give rise to the formed elements of the blood. Over the last decade, their ability to differentiate to other mesodermal cell types (e.g., fibroblasts, mesangial cells, microglia, adipocytes, chondrocytes, osteoblasts) has been broadly reported. We have previously described their contribution to homeostatic maintenance of the cardiac valves as well as to the progression of myocardial injury after myocardial infarction. Current efforts are focused on elucidation of their functional contribution to embryonic valvulogenesis and how we can use circulating cells to modulate pathological remodeling in murine models of heart disease.
- Acceleration of the assembly and maturation of small diameter bioengineered vascular grafts by improving heterotypic vascular cell interactions: A significant challenge in the biofabrication of small diameter vascular grafts is the assembly and retention of a biofunctional endothelium that delimits their inner, lumen. We hypothesize that functional retention of the endothelium can be improved through optimization of instructive interactions between the endothelium and underlying vascular smooth muscle cells; these molecular interactions are known to be important during development and remodeling of the vasculature in vivo. Toward this goal, current efforts are focused on the use of a naturally occurring nanoscaffold that promotes endothelial cell migration, assembly and barrier function to effect rapid endothelial assembly and functional interaction between the endothelium and vascular smooth muscle cells.
- Visconti RP, Awgulewitsch A. Topographic patterns of vascular disease: HOX proteins as determining factors? World J Biol Chem. 2015, Aug 26;6(3):65-70 PMID: 26322165
- Olsen TR, Mattix B, Casco M, Herbst A, Williams C, Tarasidis A, Simionescu D, Visconti RP, Alexis F. Manipulation of cellular spheroid composition and the effects on vascular tissue fusion. Acta Biomater. 2015, 13:188-98 PMID: 25463485
- Reese C, Lee R, Bonner M, Perry B, Heywood J, Silver RM, Tourkina E, Visconti RP, Hoffman S. Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry. Front Pharmacol. 2014, 5:141 PMID: 24999331 PMCID: PMC4058709
- Jia J, Richards DJ, Pollard S, Tan Y, Rodriguez J, Visconti RP, Trusk TC, Yost MJ, Yao H, Markwald RR, Mei Y. Engineering alginate as bioink for bioprinting. Acta Biomater. 2014, 10(10):4323-31 PMID: 24998183 PMCID: PMC4350909
- Lee R, Perry B, Heywood J, Reese C, Bonner M, Hatfield CM, Silver RM, Visconti RP, Hoffman S, Tourkina E. Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis. Front Pharmacol. 2014, 5:140 PMID: 24966836 PMCID: PMC4052341
- Tan Y, Richards DJ, Trusk TC, Visconti RP, Yost MJ, Kindy MS, Drake CJ, Argraves WS, Markwald RR, Mei Y. 3D printing facilitated scaffold-free tissue unit fabrication. Biofabrication. 2014, 6(2):024111 PMID: 24717646 PMCID: PMC4418504
- Mattix B, Olsen TR, Gu Y, Casco M, Herbst A, Simionescu DT, Visconti RP, Kornev KG, Alexis F. Biological magnetic cellular spheroids as building blocks for tissue engineering. Acta Biomater. 2014, 10(2):623-9 PMID: 24176725
- de Vlaming A, Sauls K, Hajdu Z, Visconti RP, Mehesz AN, Levine RA, Slaugenhaupt SA, Hagège A, Chester AH, Markwald RR, Norris RA. Atrioventricular valve development: new perspectives on an old theme. Differentiation. 2012, 84(1):103-16 PMID: 22579502 PMCID: PMC3389153
- Mehesz AN, Brown J, Hajdu Z, Beaver W, da Silva JV, Visconti RP, Markwald RR, Mironov V. Scalable robotic biofabrication of tissue spheroids. Biofabrication. 2011, 3(2):025002 PMID: 21562365
- Hajdu Z, Mironov V, Mehesz AN, Norris RA, Markwald RR, Visconti RP. Tissue spheroid fusion-based in vitro screening assays for analysis of tissue maturation. J Tissue Eng Regen Med. 2010, 4(8):659-64 PMID: 20603872 PMCID: PMC2951485
- Visconti RP, Kasyanov V, Gentile C, Zhang J, Markwald RR, Mironov V. Towards organ printing: engineering an intra-organ branched vascular tree. Expert Opin Biol Ther. 2010, 10(3):409-20 PMID: 20132061
- Mironov V, Visconti RP, Kasyanov V, Forgacs G, Drake CJ, Markwald RR. Organ printing: tissue spheroids as building blocks. Biomaterials. 2009, 30(12):2164-74 PMID: 19176247 PMCID: PMC3773699
- Visconti RP, Markwald RR. Recruitment of new cells into the postnatal heart: potential modification of phenotype by periostin. Ann N Y Acad Sci. 2006, 1080:19-33 PMID: 17132772
- Mironov V, Visconti RP, Markwald RR. American Association of Anatomists meeting on regenerative medicine. Expert Opin Biol Ther. 2006, 6(7):727-30 PMID: 16805712
- Mironov V, Kasyanov VA, Yost MJ, Visconti R, Twal W, Trusk T, Wen X, Ozolanta I, Kadishs A, Prestwich GD, Terracio L, Markwald RR. Cardiovascular tissue engineering I. Perfusion bioreactors: a review. J Long Term Eff Med Implants. 2006, 16(2):111-30 PMID: 16700652
- Visconti RP, Ebihara Y, LaRue AC, Fleming PA, McQuinn TC, Masuya M, Minamiguchi H, Markwald RR, Ogawa M, Drake CJ. An in vivo analysis of hematopoietic stem cell potential: hematopoietic origin of cardiac valve interstitial cells. Circ Res. 2006, 98(5):690-6 PMID: 16456103
- Mironov V, Visconti RP, Markwald RR. On the role of shear stress in cardiogenesis. Endothelium. 2005, 12(5-6):259-61 PMID: 16410225
- Visconti RP, Barth JL, Keeley FW, Little CD. Codistribution analysis of elastin and related fibrillar proteins in early vertebrate development. Matrix Biol. 2003, 22(2):109-21 PMID: 12782138