- 1. To perform high quality research in biofabrication
- 2. To serve as an education/training center for developing a biofabrication workforce
- 3. To develop enabling technologies for industrial biofabrication
- 4. To promote national and international collaboration in biofabrication
Early work in 3D tissue biofabrication was spearheaded by investigators at Clemson and MUSC resulting in a number of publications (1-5). These studies lead to collaborative work between MUSC and the University of Missouri with cell spheroids and spheroid fusion. A method of printing cell aggregates was developed and patented (US 8,241,905 with additional patents pending). Organovo (San Diego CA) exclusively holds the patent rights of this methodology. These early efforts and work by scientists across South Carolina resulted in a National Science Foundation (NSF) award of $20 million over five years to build biofabrication infrastructure across the state. With this award, we have moved well beyond these early stages with more than 100 state researchers from multiple disciplines and a host of state universities contributing. Those universities include Claflin, SC State, USC, Clemson, College of Charleston and Furman. Among its many accomplishments, the program has succeeded in printing microvascular structures using living cells. These structures, measuring 100 microns by 10 millimeters, mimic the design of blood vessels of tissues.
- 1. Wilson WC, Jr. & Boland T. Cell and organ printing 1: protein and cell printers. Anat Rec A Discov Mol Cell Evol Biol 2003 272: 491-496 [PMID: 12740942].
- 2. Boland T, Mironov V, Gutowska A, Roth EA & Markwald RR. Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels. Anat Rec A Discov Mol Cell Evol Biol 2003 272: 497-502 [PMID: 12740943].
- 3. Mironov V, Boland T, Trusk T, Forgacs G & Markwald RR. Organ printing: computer-aided jet-based 3D tissue engineering. Trends Biotechnol 2003 21: 157-161 [PMID: 12679063].
- 4. Mironov V. Printing technology to produce living tissue. Expert Opin Biol Ther 2003 3: 701-704 [PMID: 12880370].
- 5. Jakab K, Neagu A, Mironov V, Markwald RR & Forgacs G. Engineering biological structures of prescribed shape using self-assembling multicellular systems. Proc Natl Acad Sci U S A 2004 101: 2864-2869 [PMID: 14981244].
Sept 22, 2015 The paper "Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer" by Dennis SG, Trusk T, Richards D, Jia J, Tan Y, Mei Y, Fann S, Markwald, R, & Yost, M. was published in J Vis Exp. (PMID: 26436877)
May 13, 2015 The paper "Silicon nanowire-induced maturation of cardiomyocytes derived from human induced pluripotent stem cells" by Tan Y, Richards D, Xu R, Stewart-Clark S, Mani SK, Borg TK, Menick DR, Tian B, & Mei Y. was published in Nano Lett. (PMID: 25826336)
March, 2015 The paper "Implanted Scaffold-Free Prevascularized Constructs Promote Tissue Repair" by Czajka CA, Calder BW, Yost MJ & Drake CJ was published in Ann Plast Surg. (PMID: 25668498)
Nov 20, 2014 The paper "Manipulation of Cellular Spheroid Composition and the Effects on Vascular Tissue Fusion" by Olsen TR, Mattix B, Casco M, Herbst A, Williams C, Tarasidis A, Simionescu D, Visconti RP & Alexis F was published in Acta Biomater. (PMID: 25463485)
Nov 6, 2014 The paper "The Fusion of Tissue Spheroids Attached to Pre-stretched Electrospun Polyurethane Scaffolds" by Beachley V, Kasyanov V, Nagy-Mehesz A, Norris R, Ozolanta I, Kalejs M, Stradins P, Baptista L, da Silva K, Grainjero J, Wen X & Mironov V was published in J. Tissue Eng. (PMID: 25396042)