The Replicator 2 is a 4th generation Cartesian printer utilizing Fused Filament Fabrication technology with polylactic acid (PLA). This device is used to introduce students to 3D printing technology, and to produce parts and molds used in developing tissue biofabrication techniques. The build space is 11 x 6 x 6 inches. Printer head precision is 11 micrometers in X and Y; and 2.5 micrometers in Z. Printing Layer resolution ranges from Low (340 micrometers) to High (100 micrometers). The print head nozzle is 0.4 mm in diamter. Software and manuals for the Replicator 2 are available at the website makerbot.com

The epMotion 5070 is a 4-position automated liquid handler with three virtual positions to automate a wide array of liquid handling tasks. It is ideal for automation of qPCR, ELISA and other simple lab tasks that require a high degree of accuracy and reproducibility.The epMotion can pipette microliter volumes with < 1.9% imprecision, thus reaction volumes can be as little few microliters (pipetting range from 1 µl to 1,000 µl). Routine pipetting tasks can be automated with more precision and safety than manual pipetting. The epMotion platform lets the operator automate applications using all types of labware. The machine has 700 single-tube formats, well plates, and even unusual plastics such as real-time rotor tubes.

DynaGen Bioreactor System is a third generation bioreactor that provides mechanical stimulation to tissue engineered constructs during growth. The system consists of Growthworks Control box, a Cole Parmer Masterflex L/S pump controlled by Digital Modular Drive, a 6Vr Lumegen Bioreactor Chamber and a 67Vr Pulsatile pump stimulator.The DynaGen bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The LumeGen system imparts pulsatile flow and pressure to a vascular conduit. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices.

The ElectroForce 3100 test instrument provides 22 N of linear force with 20 G acceleration and frequency response to 100 Hz. The high resolution transducer configuration combines a 250 gram force transducer, and a 1.0mm full scale (±500µm) displacement transducer, to enhance control resolution in force and displacement. The 3100 is useful in tissue mechanics research, micro-indentation of cartilage and other soft tissues, mechanical stimulation of tissue engineered constructs, and the dynamic mechanical analysis of tissues, elastomers and other soft materials.

5940 Single Column Testing Systems are commonly used for medical devices and biomaterials, textiles, elastomers, food, small components and microelectronics, wire, paper, and plastic film. Two key advantages of 5940 single column systems are their small footprint and frame stiffness. The superior stiffness and alignment of the 5900 load frames ensures precise alignment when testing everything from biomaterials to wire or plastic film. The rigid mechanical design guarantees the best possible conditions for repeatable test conditions and reliable results. Load measurement accuracy: ±0.5% of reading down to 1/500 of load cell capacity (2580 Series load cells); up to 1 kHz data acquisition rate simultaneous on load, extension, and strain channels; and speed range of 0.05 - 2500 mm/min (0.002 - 100 in/min). (MANUAL)

The AR 1000 rheometer is a controlled stress/controlled rate rhemeter capable of handling many different types of samples, using a range of geometery sizes and types. The Torque Range is 0.1 micro Newton meters to 100 milli Newton meters. Depending on geometery, shear stress can be determined within the range of 0.0008 to 508,000 Pa. Angular displacement resolution is 0.62 microrads. The normal force range is 1 to 5000 g. A Peltier stage controls sample temperature from -10 to 99 degrees C, with a rate change of 20 degrees C per minute. (MANUAL) (Setup)

Application Note: Normal Force Measurements: Part 1 Part 2

Th Nikon TE-2000S microscope has Hoffman Modulation and Phase Contrast capabilities, and is equipped with excitation-emission filter sets for fluorescent imaging using dyes similar to FITC and Texas Red. The DV2 provides live digital viewing of combined fluorescence (FITC+Texas Red). Hoffman Modulation Contrast is useful for viewing unstained samples growing in plastic chambers. The HQ2 camera captures 14-bit images at 1392 x 1040 pixels. NIS-Elements software from NIKON is installed on the computer.

The Beckman Coulter MoFlo Astrios High Speed Cell Sorter is capable of analyzing 32 parameters; FSC, SSC, and 30 fluorescent channels. It uses 4 solid state lasers (488 nm blue laser; 642 nm red laser; 355 nm UV laser; and 561 nm yellow-green laser), and can analyzes up to 100,000 events per second. 6-way sorting at speeds of up to 70,000 events per second is also possible. The CyAn ADP Cellular Analyzer is able to analyze 11 parameters; FSC, SSC, and 9 fluorescent channels. It uses 3 solid state lasers as excitation sources: 488 nm blue laser; 642 nm red laser; and 405 nm violet laser. Up to 70,000 events per second can be analyzed. The Flow Cytometry Facility is located in BSB639. Dr. Visconti directs the Facility and Chris Fuchs manages the resources. The facility webpage has more details.

Powered by Ion Torrent™ semiconductor chip technology, the Ion Personal Genome Machine™ (PGM™) Sequencer delivers the fastest run times of any next-generation sequencer. High accuracy, and long reads put the Ion PGM™ Sequencer in a class of its own and makes next-generation sequencing more accessible. Visit the MUSC Proteogenomic Facility webpage for information on Ion Torrent base sequencing services and pricing.

The DH-40iL Microincubator is designed for imaging live cells under constant temperature and atmospheric conditions. This versatile incubation system enables high resolution image analysis, fluorescence microscopy, and time-lapse imaging studies while accommodating both upright and inverted microscopes.

The DH-40iL will work in both its open and closed configurations. The system includes a single channel heater controller and a CO₂ / pH controller.

Other Equipment
Metler Toledo XS203S Scale

Metler Toledo SevenEasy PH Meter

World Precision Instruments Dissecting Microscope

2 Flexible long neck microscope light sources (Cole Parmer and NovaFlex)

Fisher Scientific Marathon 12K Centrifuge

Olympus CK2 Inverted Microscope

Forma Scientific CO₂ Tissue Culture 37°C Incubator

Labconco Tissue Culture Hood
Baker Company Tissue Culture Hood

WPI pump (LV140-4)

VWR Water Bath

New Brunswick Platform Shaker

Cole Parmer Masterflex LS computerized drive pump

Cole Parmer Masterflex LS compact drive pump

Eppendorf 5702 Microtube Centrifuge

Equatherm Water Bath

Liquid Nitrogen Tank (47 Liter)